Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hepatic lipid quantity and quality in type 2 diabetes mellitus.

Frontiers in pharmacology·2026
Same author

Efficacy of intranasal adenovirus vector vaccine is attenuated by type I IFN-induced NK cell activation.

Molecular therapy. Advances·2026
Same author

Effects of cellular receptors on the vaccine efficacies of adenovirus vector-based vaccines following intramuscular and intranasal administration.

Scientific reports·2026
Same author

Liver-Specific Suppression of PLA2G6/iPLA<sub>2</sub>β Improves Glucose and Lipid Metabolism in High-Fat Diet-Fed Mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Extending a liver-based model to lung and intestine: a comparative study of CYP1A1 and CYP1A2 induction by cigarette smoke in human in vitro models.

Archives of toxicology·2026
Same author

Automated High-Throughput Raman Spectral Framework for Cellular Differentiation Monitoring.

Nano letters·2026

Related Experiment Video

Updated: May 26, 2026

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
12:40

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Published on: May 19, 2018

Efficient hepatic differentiation from human iPS cells by gene transfer.

Kenji Kawabata1, Mitsuru Inamura, Hiroyuki Mizuguchi

  • 1Laboratory of Stem Cell Regulation, National Institute of Biomedical Innovation, Osaka, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|December 15, 2011
PubMed
Summary

Efficiently differentiating hepatic cells from human stem cells is crucial for regenerative medicine and drug discovery. This study demonstrates a novel adenovirus vector method to improve hepatic cell differentiation from embryonic stem (ES) and induced pluripotent stem (iPS) cells.

More Related Videos

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations
05:49

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations

Published on: October 26, 2011

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
08:05

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

Published on: May 6, 2021

Related Experiment Videos

Last Updated: May 26, 2026

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
12:40

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Published on: May 19, 2018

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations
05:49

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations

Published on: October 26, 2011

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
08:05

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

Published on: May 6, 2021

Area of Science:

  • Stem cell biology
  • Hepatology
  • Regenerative medicine

Background:

  • Hepatic cell differentiation from human embryonic stem (ES) and induced pluripotent stem (iPS) cells is vital for regenerative therapies and drug development.
  • Current differentiation protocols yield poor efficiency due to complex multi-lineage pathways.
  • Improving hepatic differentiation is essential for advancing cell-based treatments and pharmaceutical research.

Purpose of the Study:

  • To develop efficient protocols for hepatic differentiation from human ES and iPS cells.
  • To overcome the limitations of existing methods for generating hepatic cells.
  • To establish a robust method for generating hepatic cells for therapeutic and research applications.

Main Methods:

  • Utilized adenovirus vector-mediated gene transfer.
  • Focused on enhancing the differentiation efficiency of endoderm-derived cells.
  • Applied protocols to both human ES and iPS cells.

Main Results:

  • Achieved efficient hepatic differentiation from human ES and iPS cells.
  • Demonstrated the effectiveness of the adenovirus vector-mediated gene transfer protocol.
  • Overcame poor differentiation efficiency observed in previous methods.

Conclusions:

  • Adenovirus vector-mediated gene transfer provides an efficient method for hepatic differentiation from human ES and iPS cells.
  • This protocol can significantly contribute to regenerative cell therapies.
  • The findings support advancements in drug discovery and development using patient-specific hepatic cells.