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Related Experiment Video

Updated: May 26, 2026

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat
13:27

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

Published on: October 6, 2018

Engineering of implantable liver tissues.

Yasuyuki Sakai1, M Nishikawa, F Evenou

  • 1Institute of Industrial Science, University of Tokyo, Tokyo, Japan. sakaiyas@iis.u-tokyo.ac.jp

Methods in Molecular Biology (Clifton, N.J.)
|December 15, 2011
PubMed
Summary

Engineered liver tissues require precise oxygen control for cell function and growth. Different configurations show promise for clinical applications, with oxygen supply being the critical engineering factor for successful tissue regeneration.

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Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Engineered liver tissues are crucial for treating liver diseases.
  • Oxygen supply is a key engineering challenge in liver tissue engineering.
  • Three configurations are explored: cell sheet-based, scaffold-based, and flow channel-based tissues.

Purpose of the Study:

  • To review engineering approaches for creating functional liver tissues.
  • To highlight the critical role of oxygen supply in different engineered liver tissue configurations.
  • To assess the feasibility of various engineered liver tissue designs for clinical applications.

Main Methods:

  • Investigated cell sheet-based tissues with oxygen-permeable membranes for controlled oxygen supply.

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Last Updated: May 26, 2026

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat
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A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

Published on: October 6, 2018

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy
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  • Utilized poly-L-lactic acid scaffolds with growth factors for sheet-like macroporous tissue engineering.
  • Developed design criteria for flow channel networks in scaffold-based tissues considering oxygen diffusion.
  • Evaluated cell behavior, growth, and maturation under controlled oxygen conditions in vitro and in vivo.

    • Main Results:

    • Cell sheet-based tissues demonstrated mature cell layers and tissue formation with controlled oxygen.
    • Scaffold-based tissues showed in vitro maturation, with proliferation upon implantation.
    • Flow channel-based tissues achieved lower cell densities than in vivo, indicating a need for improved fabrication.
    • Optimal oxygen levels were found to be crucial for cell respiration and reorganization.

    Conclusions:

    • Oxygen supply is the most critical factor in engineering liver tissues across all configurations.
    • Cell sheet and scaffold-based approaches show promise for clinical translation in liver disease therapy.
    • Advancements in 3D microfabrication are needed for complex, high-density engineered tissues.
    • Controlled oxygenation enhances cellular respiration and tissue development, supporting regenerative medicine goals.