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Synthesis and characterization of oligodeoxynucleotides containing the mutagenic base analogue 4-O-ethylthymine
D Fernandez-Forner1, Y Palom, S Ikuta
1Department of Organic Chemistry, University of Barcelona, Spain.
Nucleic Acids Research
|October 11, 1990
Abstract:
A method for the preparation of oligonucleotides containing the mutagenic base 4-O-ethylthymine is described for the first time. Use of p-nitrophenylethyl type base protecting groups together with phosphitetriester solid-phase methodology makes possible the rapid and efficient preparation of oligonucleotides bearing 4-O-ethylthymine, while standard base protecting groups are not compatible with the presence of this base. Possible applications of this methodology are discussed.