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Updated: May 26, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
Assignment of backbone resonances in a eukaryotic protein kinase - ERK2 as a representative example
Andrea Piserchio1, Kevin N Dalby, Ranajeet Ghose
1Department of Chemistry, The City College of New York, New York, NY, USA.
None:
A first step toward the analysis of the structure, dynamics, and interactions of proteins by NMR is obtaining an acceptable level of resonance assignments. This process is nontrivial in most eukaryotic kinases given their size and suboptimal behavior in solution. Using inactive ERK2 as a representative example, we describe the procedures we utilized to achieve a significant degree of completeness of backbone resonance assignment.
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