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Updated: May 26, 2026
Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
Published on: July 21, 2023
Increasing surface enhanced Raman spectroscopy effect of RNA and DNA components by changing the pH of silver
Oliva M Primera-Pedrozo1, Gabriela Del Mar Rodríguez, Jorge Castellanos
1Center for Sensor Development/Chemical Imaging Center/ALERT DHS-COE, Department of Chemistry, University of Puerto Rico-Mayaguez, P.O. Box 9019, Mayaguez, PR 00681, Puerto Rico. olivaprimera@yahoo.es
Abstract:
This work focused on establishing the parameters for enhancing the Raman signals of DNA and RNA constituents: nitrogenous bases, nucleosides and nucleotides, using metallic nanoparticles as surface enhanced Raman scattering substrates. Silver nanospheres were synthesized using sodium borohydride as a reducing agent and sodium citrate as a capping agent. The prepared nanoparticles had a surface plasmon band at ∼384nm and an average size of 12±3nm. The nanoparticles' surface charge was manipulated by changing the pH of the Ag colloidal suspensions in the range of 1-13. Low concentrations as 0.7μM were detected under the experimental conditions. The optimum pH values were: 7 for adenine, 9 for AMP, 5 for adenosine, 7 for dAMP and 11 for deoxyadenosine.
