Related Experiment Video
Updated: May 26, 2026
Murine Model of Intestinal Ischemia-reperfusion Injury
Published on: May 11, 2016
[Effect of ischemic postconditioning on oxidative stress and structural tissue changes in intestinal warm ischemic
Klára Nedvig1, Edina Völgyi, György Wéber
1Zala Megyei Kórház, Általános Sebészeti Osztály, Zalaegerszeg. nedvigklari@gmail.com
Introduction/Aim:
Our study investigated the effect of ischemic postconditioning (IPO) in intestinal warm ischemia/reperfusion (I/R) and autotransplantation models.
Materials And Methods:
Warm ischemia was performed by occlusion of superior mesenteric artery for 1, 3 and 6 hours in white domestic pigs (n = 15). Prior to 3 hours reperfusion the intestine was postconditioned by 3 cycles of 30-seconds ischemia and 30-seconds reperfusion (IPO protocol). In the cold ischemia group (n = 15) the bowel was preserved in University of Wisconsin solution for 1, 3, and 6 hours. Prior to 3 hours reperfusion IPO protocol was applied, too. Tissue samples were collected after laparotomy (control) and at the end of the reperfusion periods. As far as oxidative stress markers, malondialdehyde and reduced glutathione (GSH) levels and superoxide dismutase (SOD) activity were determined. Tissue damage was evaluated by qualitative (Park-classification) and quantitative (Scion Image) methods.
Results:
As regards oxidative stress parameters, lipidperoxidation decreased and the protective effect of endogenous antioxidants (GSH, SOD) retained significantly by IPO procedure at the end of reperfusion. Tissue injury correlated significantly by the duration of warm ischemia and cold preservation. Quantitative analysis demonstrated that IPO ameliorated tissue injury in each group (p < 0.05).
Conclusion:
IPO significantly attenuated intestinal oxidative stress and morphological damages in warm and cold I/R models.
