Related Experiment Video
Updated: May 26, 2026
Disruption of the Mouse Blood-Brain Barrier by Small Extracellular Vesicles from Hypoxic Human Placentas
Published on: January 26, 2024
[Role of perforin in severe preeclampsia]
1Department of Obstetrics and Gynecology, Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, China. weij@sj-hospital.org
Objectives:
To investigate the possible role of perforin (PFN) in the pathogenesis of severe preeclampsia.
Methods:
Thirty-two cases of severe preeclampsia were included in the study. Thirty-two cases of normal pregnancy were selected as control group in random. The expression of PFN mRNA in the peripheral blood mononuclear cells (PBMC) was detected by reverse transcription (RT)-PCR, and its correlation with mean arterial pressure was analyzed in severe preeclamptic patients. The expression of PFN protein in the decidua was detected by immunohistochemistry.
Results:
(1) The expression of PFN mRNA in PBMC:the PFN mRNA level in severe preeclamptic group was 1.19 ± 0.31, and that in normal pregnancy group is 0.82 ± 0.28. The PFN mRNA level in severe preeclamptic group was significantly higher than that of control group (P < 0.01). (2) Correlation analysis: the mean blood pressure in severe preeclampsia group was (133 ± 5) mm Hg (1 mm Hg = 0.133 kPa). There was significant positive correlation between level of PFN mRNA in PBMC and mean blood pressure in severe preeclamptic patients (r = 0.701, P = 0.000). (3) Decidual PFN protein expression:PFN protein was mainly expressed in lymphocytes and the cytoplasm of decidual stromal cells. The positive ratio of PFN in the decidua of severe preeclamptic patients was 84% (27/32), significantly higher than that of control group (53%, 17/32, P < 0.01).
Conclusions:
Expression of PFN was significantly increased in severe preeclampsia, and it was of significant positive correlation with mean blood pressure. PFN may participate in the pathogenesis of severe preeclampsia.
