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Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
10:46

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Cardiomyocytes rhythmically beating generated from goat embryonic stem cell.

S Garg1, R Dutta, D Malakar

  • 1Animal Biotechnology Center, National Dairy Research Institute, Karnal-132001, India.

Theriogenology
|December 16, 2011
PubMed
Summary
This summary is machine-generated.

Researchers successfully isolated and characterized goat embryonic stem cells (ES cells) from in vitro fertilized embryos. These goat ES cells were differentiated into beating cardiomyocytes, demonstrating potential for regenerative medicine applications.

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Area of Science:

  • Reproductive Biology and Stem Cell Science
  • Developmental Biology and Regenerative Medicine

Background:

  • Embryonic stem cells (ES cells) hold significant promise for regenerative medicine due to their pluripotency.
  • Establishing and characterizing ES cells from domestic animals like goats is crucial for advancing comparative stem cell research and potential therapeutic applications.

Purpose of the Study:

  • To isolate and characterize putative goat embryonic stem cells (ES cells) derived from in vitro fertilized (IVF) goat embryos.
  • To differentiate these goat ES cells into functional cardiomyocytes and assess their potential for cardiac tissue regeneration.

Main Methods:

  • Goat blastocysts were produced using in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC).
  • Inner cell masses (ICMs) were isolated and cultured on a feeder layer, with characterization using immunocytochemistry and RT-PCR for specific markers (Oct4, Sox2, Nanog, SSEA-4, TRA-1-81).
  • Embryoid bodies (EBs) were generated and differentiated using specific growth factors (Activin-A, FGF-2, BMP-4), followed by analysis of cardiac markers (α Actinin, C-Troponin).

Main Results:

  • Putative goat ES cells were successfully isolated, cultured up to the 22nd passage (with feeder) and 12th passage (without feeder), maintaining pluripotency markers.
  • Normal karyotyping was confirmed, and the cells expressed key surface and intracellular markers characteristic of ES cells.
  • Differentiated embryoid bodies exhibited rhythmic contractions indicative of cardiomyocytes, positive for cardiac-specific markers, and maintained beating for over 160 days.

Conclusions:

  • The study successfully established and characterized goat ES cells capable of long-term culture and maintenance of pluripotency.
  • A robust differentiation protocol was developed, enabling the generation of functional, beating cardiomyocytes from goat ES cells.
  • These findings highlight the potential of goat ES cells for applications in cardiac regenerative medicine and comparative stem cell biology.