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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Related Experiment Video

Updated: May 26, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Mass spectrometry-based proteomics for systems biology.

Eduard Sabidó1, Nathalie Selevsek, Ruedi Aebersold

  • 1Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Wolfgang-Pauli-Strasse 16, 8093 Zurich, Switzerland.

Current Opinion in Biotechnology
|December 16, 2011
PubMed
Summary

Mass spectrometry (MS)-based proteomics is crucial for systems biology, identifying and quantifying molecular interactions in dynamic biological networks. This review highlights MS

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Area of Science:

  • Biochemistry and Molecular Biology
  • Systems Biology
  • Proteomics

Background:

  • Systems biology investigates biological processes as dynamic molecular networks.
  • Mass spectrometry (MS)-based proteomics is a key technology in systems biology.
  • Understanding molecular interactions is essential for comprehending biological systems.

Purpose of the Study:

  • To review recent advancements in MS-based proteomics for systems biology.
  • To illustrate the role of MS in elucidating molecular network components and interactions.
  • To highlight the significance of MS in quantifying network perturbations.

Main Methods:

  • Review of recent literature on MS-based proteomics applications in systems biology.
  • Analysis of case studies demonstrating MS capabilities.
  • Discussion of MS's role in identifying and quantifying molecular components.

Main Results:

  • MS-based proteomics has significantly advanced systems biology research.
  • MS enables comprehensive identification of biological system components.
  • MS is vital for quantifying the effects of perturbations in molecular networks.

Conclusions:

  • Mass spectrometry is indispensable for systems biology.
  • MS facilitates a deeper understanding of complex molecular networks.
  • Continued development of MS techniques will further drive systems biology innovation.