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Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

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Published on: July 20, 2022

Physical analysis of virus particles using electrospray differential mobility analysis.

Leonard F Pease¹

¹Department of Chemical Engineering, University of Utah, Salt Lake City, UT 84112, USA. pease@eng.utah.edu

Trends in Biotechnology

|December 17, 2011

Summary

Electrospray differential mobility analysis (ES-DMA) offers precise viral size and concentration measurements for biomanufacturing. This versatile technology aids in viral clearance, identification, and bionanoconjugate preparation.

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Area of Science:

Biotechnology
Nanotechnology
Virology

Background:

Accurate measurement of viral particles is crucial in biomanufacturing and diagnostics.
Existing methods for viral analysis can be limited in precision or scope.

Purpose of the Study:

To critically review electrospray differential mobility analysis (ES-DMA) as a tool for viral analysis.
To highlight the capabilities and future applications of ES-DMA in various scientific fields.

Main Methods:

Electrospray differential mobility analysis (ES-DMA) for simultaneous size and concentration determination.
Analysis of size distributions from individual capsid proteins to intact viral particles with subnanometer precision.

Main Results:

ES-DMA provides full multimodal size distributions with high precision.
The technology enables simultaneous determination of viral size and concentration.

Conclusions:

ES-DMA is a powerful tool for viral clearance, monitoring, identification, and bionanoconjugate preparation.
Future applications include at-line process sensing, bioseparating, and studying virus-host interactions.