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Of mice and microflora: considerations for genetically engineered mice
P M Treuting1, C B Clifford, R S Sellers
1Department of Comparative Medicine & Histology and Imaging Core, School of Medicine, University of Washington, T140 Health Science Center, Box 357190, Seattle, WA 98195-7190, USA. treuting@uw.edu
Mouse microflora significantly influences genetically engineered mouse (GEM) phenotypes. Understanding these host-microbe interactions is crucial for accurate research and developing new disease models.
Area of Science:
- Microbiology
- Genetics
- Immunology
Background:
- Genetically engineered mouse (GEM) phenotypes result from genetic, environmental, and microflora interactions.
- Host-microbe interactions, often overlooked, can lead to novel model systems and microbial discoveries.
- Historically, research focused on disease-causing microbes, but GEMs reveal previously unknown agents.
Purpose of the Study:
- To illustrate how microflora impacts GEM phenotypes.
- To highlight infectious diseases in GEM colonies.
- To review underappreciated microbial agents affecting mouse models.
Main Methods:
- Review of infectious diseases in GEM colonies.
- Analysis of host-microbe interactions in GEMs.
- Literature review of relevant microbial agents.
Main Results:
- Microflora plays a critical role in shaping GEM phenotypes.
- GEMs have revealed novel microbial agents impacting research.
- Understanding microflora is essential for interpreting GEM data.
Conclusions:
- Microflora is a key determinant of GEM phenotypes.
- Host-microbe interactions are vital for translational research using GEM models.
- Further investigation into microflora's role is necessary for advancing biomedical research.
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