Related Experiment Video

Updated: May 26, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Mammary gland selective excision of c-jun identifies its role in mRNA splicing

Sanjay Katiyar1, Xuanmao Jiao, Sankar Addya

  • 1Department of Cancer Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.

Cancer Research
|December 17, 2011
PubMed

Insights

The c-jun gene acts as an inhibitor of RNA splicing, impacting cellular processes. This finding is crucial for understanding cancer development and therapy resistance.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Biology

Background:

  • The c-jun gene regulates cellular proliferation and apoptosis through gene expression.
  • Alternative splicing of pre-mRNA diversifies protein functions and is implicated in tumor development.

Purpose of the Study:

  • To investigate the role of the endogenous c-jun gene in RNA splicing within the mouse mammary epithelium.
  • To identify specific genes and pathways regulated by c-jun in the context of splicing.

Main Methods:

  • Targeted excision of the endogenous c-jun gene in mouse mammary epithelium.
  • Microarray-based gene expression analysis of c-jun(-/-) mammary epithelium.
  • Genome-wide splicing array analysis.

Main Results:

  • Endogenous c-jun was identified as a selective inhibitor of RNA splicing.
  • Approximately 50 RNA splicing-governing genes were found to be regulated by endogenous c-jun.
  • Endogenous c-jun regulated alternative exon splicing in approximately 147 genes, with 18% linked to human tumors or apoptosis.
  • Reduced splicing activity was observed, dependent on the c-jun dimerization domain.

Conclusions:

  • Endogenous c-jun directly attenuates RNA splicing efficiency.
  • Alternative splicing's role in cancer development and therapy resistance highlights the biologic importance of these findings.

More Related Videos

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...