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Updated: May 26, 2026
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Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
Published on: January 7, 2019
Measurement of plasma volume using fluorescent silica-based nanoparticles
Christoph Eisner1, Hooisweng Ow, Tianxin Yang
1National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Journal of Applied Physiology (Bethesda, Md. : 1985)
|December 17, 2011
Summary
This study introduces fluorescent nanoparticles for accurate plasma volume measurement, overcoming limitations of traditional dye methods. This nanotechnology offers a reliable approach for clinical assessment of hemodynamic changes.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Cardiovascular Physiology
Background:
- Plasma volume (PV) is crucial for cardiovascular function and organ perfusion.
- Current PV measurement methods using tracer dilution have significant drawbacks.
- Accurate PV assessment is vital for managing various clinical conditions.
Purpose of the Study:
- To evaluate fluorescent nanoparticles as a novel tool for measuring plasma volume.
- To overcome the limitations associated with traditional dye-based tracer dilution methods.
- To establish a nanotechnology-based solution for routine PV measurement.
Main Methods:
- Synthesized size-gradated fluorescent nanoparticles with a silica core and PEG shell.
- Determined renal filtration cutoff for nanoparticles in mice (≤11 nm).
- Correlated nanoparticle-based PV measurements with Evans blue dye and assessed PV changes with albumin/furosemide.
Main Results:
- Nanoparticles were optically stable in plasma and unaffected by albumin.
- Established a linear relationship between body weight and PV using 20-30 nm nanoparticles.
- Demonstrated successful induction of PV expansion/contraction and validated nanoparticle measurements against Evans blue dye.
Conclusions:
- Fluorescent nanoparticles provide a viable and accurate method for plasma volume measurement.
- This nanotechnology addresses key methodological issues in PV assessment.
- The findings support the clinical utility of nanoparticles for monitoring hemodynamic changes.
