Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nicotine biosynthesis is completed by cryptic activating glucosylation.

Nature communications·2026

Same author

Peptide modification <i>via</i> a mild carbonylative Suzuki-Miyaura reaction gives late-stage access to diaryl-ketones.

Chemical science·2026

Same author

Expediated synthesis of <i>N</i>-acyl-<i>N</i>-alkyl sulfonamide probes for protein proximity labelling.

Organic & biomolecular chemistry·2026

Same author

Live-cell 3D-SIM of Rift Valley fever virus NSs filaments reveals a polygon web architecture.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Biocompatible ligand balancing in transition metal coordination enables benign in-cell protein arylation.

Nature chemistry·2026

Same author

Site-Selective Protein Modification via Peptide-Directed Proximity Catalysis.

ACS omega·2026

Same journal

Linker Engineering toward NIR-II Metal-Organic Framework with Maximal Emission beyond 1000 nm for Inflammatory Bowel Disease Imaging.

Journal of the American Chemical Society·2026

Same journal

Observing Kinetic Selectivity in Anthracene Photodimerization through Selective Quenching by Excited States of Proximate Rare Earth Cations.

Journal of the American Chemical Society·2026

Same journal

Sequence-Dependent Folding of Recognition-Encoded Melamine Oligomers.

Journal of the American Chemical Society·2026

Same journal

Large Thermo- and Mechanosalient Actuation via Cooperative Twist Elasticity-Induced Packing Motif Conversion.

Journal of the American Chemical Society·2026

Same journal

Discovery and Biosynthesis of Lanthipeptides Featuring an Azepinoindole Scaffold by Radical <i>S</i>-Adenosylmethionine Enzyme-Catalyzed C-C Bond Formation.

Journal of the American Chemical Society·2026

Same journal

Enantiopurity-Controlled Magnetism in a Two-Dimensional Organic-Inorganic Material.

Journal of the American Chemical Society·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 26, 2026

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Published on: November 26, 2008

Palladium-mediated cell-surface labeling.

Christopher D Spicer¹, Therese Triemer, Benjamin G Davis

¹Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Mansfield Road, Oxford OX1 3TA, UK.

Journal of the American Chemical Society

|December 20, 2011

Summary

This summary is machine-generated.

Suzuki-Miyaura coupling enabled benign C-C bond formation on bacterial cell surfaces using unnatural amino acids. This site-selective method shows promise for in vivo applications in complex organisms due to its non-toxic catalyst.

More Related Videos

Sample Preparation for Mass Cytometry Analysis

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Published on: September 7, 2019

Related Experiment Videos

Last Updated: May 26, 2026

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Published on: November 26, 2008

Sample Preparation for Mass Cytometry Analysis

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Published on: September 7, 2019

Area of Science:

Biochemistry
Synthetic Biology
Catalysis

Background:

Cell-surface engineering is crucial for various biological applications.
Developing selective and biocompatible chemical modification methods is essential.

Purpose of the Study:

To achieve site-selective C-C bond formation on the cell surface.
To explore the use of genetically incorporated unnatural amino acids in catalysis.
To assess the biocompatibility of the catalytic system for potential in vivo applications.

Main Methods:

Genetically incorporating unnatural amino acids with aryl halide side chains into *Escherichia coli*.
Utilizing Suzuki-Miyaura coupling with a phosphine-free palladium catalyst for C-C bond formation.
Evaluating cell viability post-catalysis at required palladium loadings.

Main Results:

Successful benign C-C bond formation at genetically defined sites on the *E. coli* cell surface.
The phosphine-free catalyst demonstrated no significant cell death at effective palladium concentrations.
Site-selective cell surface manipulation was achieved without compromising cell viability.

Conclusions:

Suzuki-Miyaura coupling of unnatural amino acids provides a robust method for site-specific cell surface functionalization.
The developed catalytic system is biocompatible, paving the way for in vivo applications.
This approach holds potential for future catalytic metal-mediated bond formation in more complex organisms.