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Updated: May 26, 2026

Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells
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Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells

Published on: October 20, 2023

Optimizing cord blood sample cryopreservation.

David T Harris1

  • 1Department of Immunobiology, University of Arizona, Tucson, Arizona 85724, USA. davidh@email.arizona.edu

Cytotherapy
|December 20, 2011
PubMed
Summary
This summary is machine-generated.

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Cord blood (CB) cryopreservation in albumin or plasma yields equivalent cell recovery and viability. Overwrapping CB units prevents cross-contamination during liquid nitrogen storage, ensuring sample integrity.

Area of Science:

  • Biotechnology
  • Regenerative Medicine
  • Hematology

Background:

  • Cord blood (CB) banking is increasingly prevalent globally.
  • Storing CB in multiple aliquots maximizes sample utility.
  • Optimal cryopreservation and storage methods for CB aliquots require investigation.

Purpose of the Study:

  • To analyze variables affecting cord blood cryopreservation and storage.
  • To compare different cryopreservation media and storage containers.
  • To assess methods for preventing cross-contamination during storage.

Main Methods:

  • Cord blood mononuclear cells (MNC) were frozen in human serum albumin (HSA) or autologous plasma.
  • Cryopreservation was performed using cryovials and cryobags.
  • Bacteriophage phiX174 was used to model viral cross-contamination.

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Main Results:

  • Cryopreservation in HSA, undiluted, or 50% diluted plasma showed equivalent cell recovery and viability.
  • Cryovials and cryobags exhibited similar thermal characteristics for CB sample cryopreservation.
  • Overwrapping storage containers prevented viral cross-contamination in liquid nitrogen.

Conclusions:

  • Cord blood can be cryopreserved in vials or bags with no impact on temperature stability.
  • Sample overwrapping effectively prevents microbial contamination during liquid-phase storage in liquid nitrogen.