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Related Experiment Video

Updated: May 26, 2026

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy
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Published on: April 13, 2022

Odontoblast RNA stability in different temperature-based protocols for tooth storage.

M C M Conde1, F Nedel, V F Campos

  • 1Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil.

International Endodontic Journal
|December 20, 2011
PubMed
Summary

Storing human dental pulp and pre-dentine cells at various temperatures (including room temperature and 4°C) effectively preserves RNA quality for downstream analysis, enabling endodontic research.

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Area of Science:

  • Biomaterials Science
  • Molecular Biology
  • Dental Research

Background:

  • RNA integrity is crucial for molecular studies in endodontics.
  • Human dental pulp and pre-dentine cells are valuable sources for research.
  • Standardized methods for preserving these cells are needed.

Purpose of the Study:

  • To assess the impact of four different tooth storage temperatures on RNA quality.
  • To determine the suitability of preserved dental pulp and pre-dentine cells for RT-PCR analysis.

Main Methods:

  • RNA was extracted from human dental pulp and pre-dentine cells using TRIzol reagent.
  • Teeth were stored at 4°C (6h), 20°C (24h), -80°C (24h), or in liquid nitrogen (24h).
  • RNA integrity was assessed via ribosomal RNA density, and gene expression (DSPP, DMP1, MEPE, GAPDH) was analyzed by RT-PCR.

Main Results:

  • All tested storage conditions maintained RNA integrity.
  • Odontoblast markers (DSPP, DMP1, MEPE) were successfully amplified from both pulp and pre-dentine cells.
  • GAPDH, a housekeeping gene, was also detected, confirming RNA suitability for analysis.

Conclusions:

  • Four tooth storage temperature methods preserve RNA quality for RT-PCR.
  • These findings support the use of clinically sourced human dental pulp and odontoblasts in endodontic research.
  • This research may enhance the utility of dental tissues in regenerative endodontics and personalized medicine.