Related Experiment Video
Updated: May 26, 2026
Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint
Published on: July 22, 2021
Levels of reactive oxygen metabolites in patients with knee osteoarthritis
Kazuhiko Kotani1, Naoki Sakane, Munetada Kamimoto
1Department of Clinical Laboratory Medicine, Jichi Medical University, Tochigi, Japan. kazukotani@jichi.ac.jp
Aim:
Oxidative stress may be associated with the pathophysiology of knee osteoarthritis (OA), a health-care issue. The present study aimed to compare oxidative status levels of patients with and without knee OA, using the Reactive Oxygen Metabolites (d-ROMs, Diacron, Italy) test, which has proved to be clinically useful as an oxidative stress marker.
Methods:
Forty-nine women (mean age 71.3 ± 5.8 years, mean body mass index 24.4 ± 2.2 kg/m(2) ) with mildly severe knee OA and 49, without knee OA, female controls' serum d-ROMs concentrations were assessed together with mean blood pressure, fasting plasma glucose and serum total cholesterol concentrations, using the age-, sex- and body mass index-matched method.
Results:
Patients with knee OA showed significantly higher d-ROMs levels than controls (338 ± 56 vs 315 ± 47 U. Carr., P < 0.05). This difference remained statistically significant after adjusting for other clinical variables.
Conclusions:
The present results suggested that knee OA might be an impaired oxidative condition. Further studies are necessary to establish the knee OA-oxidative status relationship, which may lead to its clinical application in the management of knee OA.
