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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

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Related Experiment Video

Updated: May 26, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

The intricate relationship between RNA structure, editing, and splicing.

Leila E Rieder1, Robert A Reenan

  • 1Department of Molecular Biology, Cellular Biology and Biochemistry, Brown University, Providence, RI 02912, USA. Leila_Rieder@brown.edu

Seminars in Cell & Developmental Biology
|December 20, 2011
PubMed
Summary

Adenosine-to-inosine RNA editing recodes genetic information via single nucleotide changes. This study explores RNA editing substrates and their interaction with splicing, clarifying their relationship.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Post-transcriptional modifications like RNA editing and splicing diversify the proteome.
  • Adenosine-to-inosine (A-to-I) RNA editing recodes RNA sequences through single nucleotide changes.
  • A-to-I editing involves the conversion of adenosine to inosine, which is read as guanosine.

Purpose of the Study:

  • To investigate the relationship between RNA editing and splicing.
  • To identify commonalities among RNA editing substrates.
  • To predict the interaction between RNA editing and splicing based on substrate characteristics.

Main Methods:

  • Analysis of RNA editing substrates.
  • Comparative analysis of editing and splicing processes.
  • Bioinformatic predictions of RNA-DNA/RNA-RNA duplex formation.

Main Results:

  • Identification of general commonalities in RNA editing substrates.
  • Development of predictions regarding the interplay between RNA editing and splicing.
  • Discussion of anomalous cases that challenge current understanding.

Conclusions:

  • RNA editing and splicing are key post-transcriptional processes that expand proteomic diversity.
  • The precise temporal relationship between RNA editing and splicing is complex and influenced by substrate features.
  • Further research is needed to fully elucidate the intricacies of RNA editing and splicing interactions.