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Related Concept Videos

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Related Experiment Video

Updated: May 26, 2026

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection
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Published on: February 16, 2020

Cellular iron distribution in Bacillus anthracis.

Wang Yung Tu1, Susanne Pohl, Joe Gray

  • 1Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom.

Journal of Bacteriology
|December 20, 2011
PubMed
Summary
This summary is machine-generated.

Bacillus anthracis intracellular iron distribution was studied, identifying ferredoxin, miniferritin Dps2, and superoxide dismutase (SOD) enzymes as major iron pools. SodA1 is cambialistic (manganese or iron), while SodA2 binds only iron.

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Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
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Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
06:37

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Published on: February 7, 2013

Area of Science:

  • Microbiology
  • Biochemistry
  • Pathogen Research

Background:

  • Iron acquisition is crucial for bacterial pathogen survival within hosts.
  • Intracellular iron distribution in bacterial pathogens remains poorly understood.

Purpose of the Study:

  • To investigate the cytosolic iron distribution in the bacterial pathogen Bacillus anthracis.
  • To identify key iron-binding proteins within Bacillus anthracis.

Main Methods:

  • Anaerobic native liquid chromatography
  • Inductively coupled plasma mass spectrometry
  • Peptide mass fingerprinting
  • In vitro assays with recombinant proteins

Main Results:

  • Identified major iron pools associated with ferredoxin, miniferritin Dps2, and superoxide dismutase (SOD) enzymes (SodA1 and SodA2).
  • SodA1 is cambialistic, utilizing manganese or iron cofactors.
  • SodA2 exclusively binds iron in vivo and is active with an iron cofactor.
  • Bacillus anthracis exposed to superoxide stress doubles its iron content, with acquired iron not localizing to the identified pools.

Conclusions:

  • Elucidated key cytosolic iron-binding proteins in Bacillus anthracis.
  • Demonstrated differential cofactor utilization by SOD isozymes SodA1 and SodA2.
  • Highlighted dynamic iron uptake in response to oxidative stress, with uncharacterized localization.