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Updated: May 26, 2026
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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
FRET-mediated pH-responsive dual fluorescent nanoparticles prepared via click chemistry
Karima Ouadahi1, Kamal Sbargoud, Emmanuel Allard
1Institut Lavoisier de Versailles UMR-CNRS 8180, Université de Versailles-Saint-Quentin-en-Yvelines, 45 Avenue des Etats-Unis, 78035 Versailles Cedex, France.
Nanoscale
|December 20, 2011
Abstract:
Herein, we report an easy preparation of azide-coated polystyrene-based nanoparticles (15 nm in diameter) and their surface functionalization via CuAAC with fluorophores in water. Resultant dual fluorescent nanoparticles coated with dansyl and pH-sensitive fluorescein moieties as the donor/acceptor FRET pair show a ratiometric response to pH upon excitation at a single wavelength.
