Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nanoscale opportunities in extracellular matrix mimicry.

Nanoscale horizons·2026

Same author

Dynamic, Reconfigurable, and Hierarchical Biosynthetic Composites via Collagen Self-Assembly within Highly Crowded Microgel Pastes.

Advanced materials (Deerfield Beach, Fla.)·2025

Same author

Assembly of non-close-packed poly(N-isopropylacrylamide) microgels on metal halide perovskite films.

Journal of colloid and interface science·2025

Same author

Real-world implementation of a noninvasive, AI-augmented, anemia-screening smartphone app and personalization for hemoglobin level self-monitoring.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

Ultrasoft platelet-like particles stop bleeding in rodent and porcine models of trauma.

Science translational medicine·2024

Same author

Highly swelling pH-responsive microgels for dual mode near infra-red fluorescence reporting and imaging.

Nanoscale advances·2022

Same journal

Innate Immunity of Framework Nucleic Acids.

Accounts of chemical research·2026

Same journal

High-Performance CH-Series Non-Fullerene Acceptors for Organic Photovoltaics.

Accounts of chemical research·2026

Same journal

Design Principles for Negative Thermal Expansion in Two-Dimensional Materials.

Accounts of chemical research·2026

Same journal

Main Group Redox Catalysis: New Frontiers with Germanium and Tin.

Accounts of chemical research·2026

Same journal

Taming Irreversibility in sp<sup>2</sup>-Carbon-Conjugated COFs from Polycrystalline Powders to Single Crystals and Thin Films.

Accounts of chemical research·2026

Same journal

Electroactive Imidazolium Ionic Liquids in Organic Synthesis.

Accounts of chemical research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 26, 2026

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

Multifunctional nanogels for siRNA delivery.

Michael H Smith¹, L Andrew Lyon

¹School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, 30332-0400, United States.

Accounts of Chemical Research

|December 21, 2011

Summary

This summary is machine-generated.

Hydrogel nanoparticles (nanogels) offer a versatile platform for delivering small interfering RNA (siRNA) therapeutics. These multifunctional nanogels overcome biological barriers for effective cancer treatment, demonstrating promise in drug-resistant ovarian cancer.

More Related Videos

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

Related Experiment Videos

Last Updated: May 26, 2026

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

Area of Science:

Biotechnology
Materials Science
Nanomedicine

Background:

RNA interference (RNAi) using small interfering RNA (siRNA) shows promise for cancer treatment but faces challenges due to poor pharmacological properties and delivery hurdles.
Nanoparticle carriers are crucial for encapsulating, protecting, and delivering siRNA to target cells, necessitating versatile designs that can overcome biological barriers.

Purpose of the Study:

To investigate hydrogel nanoparticles (nanogels) as advanced delivery vehicles for siRNA therapeutics.
To explore the design and application of multifunctional nanogels with tunable properties for enhanced drug delivery.

Main Methods:

Development of higher-order nanogel architectures using multiple, orthogonally layered hydrogel chemistries.
Functionalization of nanogel compartments for specific applications such as cell targeting, immune evasion, and therapeutic payload regulation.
Proof-of-principle experiments demonstrating siRNA delivery to drug-resistant ovarian cancer cells using core/shell nanogel structures.

Main Results:

Multifunctional nanogels can be engineered with distinct compartments, allowing independent tuning of properties for optimized delivery.
Core/shell nanogels successfully delivered siRNA to chemosensitize drug-resistant ovarian cancer cells.
Ongoing research focuses on advanced structures like biodegradable nanogels and multicompartment spheres, alongside studies on behavior in confined environments.

Conclusions:

Nanogels represent a versatile platform technology for siRNA delivery, enabling the development of multifunctional carriers with tailored properties.
Engineered nanogels can overcome biological barriers and effectively deliver therapeutic siRNA, showing significant potential in treating challenging diseases like drug-resistant cancers.