Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effect of Heat Treatment on the Corrosion and Wear Behavior of Hastelloy C276 Alloy Fabricated via Laser Powder Bed Fusion.

Materials (Basel, Switzerland)·2026

Same author

Artesunate suppresses ocular inflammation in endotoxin-induced uveitis through inhibiting the NF-κB signaling pathway and M1 macrophage polarization.

European journal of pharmacology·2025

Same author

Phytochemical and in vitro antimicrobial and antidiabetical activities of Cordiera sessilis stem bark.

Anais da Academia Brasileira de Ciencias·2025

Same author

Improving Steam Methane Reforming Efficiency via Hierarchical Structure in Additively Manufactured Ni-Based Self-Catalytic Reactors.

Materials (Basel, Switzerland)·2025

Same author

Phytochemical Profiling by HPLC-ESI-MS/MS and In Vitro Investigation of the Antidiabetic Activity of Cassia bakeriana Bark Extract and Fractions.

Journal of mass spectrometry : JMS·2024

Same author

Curcumin Attenuates Doxorubicin-Induced Cardiac Oxidative Stress and Increases Survival in Mice.

Pharmaceutics·2024

Search research articles

Related Experiment Video

Updated: May 26, 2026

Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9

Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9

Published on: January 30, 2020

Worker honeybee brain proteome.

Liudy G Hernández¹, Bingwen Lu, Gabriel C N da Cruz

¹Mass Spectrometry Group, Physics Department, CEADEN, Havana, Cuba.

Journal of Proteome Research

|December 21, 2011

Summary

This study mapped honeybee brain proteins, revealing distinct proteomes for nurse and forager bees. Nurses showed higher expression of translation-related proteins, while foragers prioritized energy production proteins.

More Related Videos

Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

Published on: September 14, 2018

Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System

Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System

Published on: September 6, 2016

Related Experiment Videos

Last Updated: May 26, 2026

Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9

Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9

Published on: January 30, 2020

Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

Published on: September 14, 2018

Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System

Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System

Published on: September 6, 2016

Area of Science:

Proteomics
Neurobiology
Insect Science

Background:

The honeybee brain proteome is crucial for understanding caste-specific behaviors.
Previous studies have provided limited insights into the proteomic differences between honeybee subcastes.

Purpose of the Study:

To conduct a large-scale proteomic analysis of the worker honeybee brain.
To identify differentially expressed proteins between nurse and forager honeybee subcastes.
To categorize identified proteins based on function using the EuKaryotic Orthologous Groups (KOG) catalog.

Main Methods:

Mass spectrometry-based proteomics, specifically MudPIT (multidimensional protein identification technology).
Bioinformatic analysis including BLASTX for sequence comparison and G-test for statistical analysis of differential expression.
Comparison with previous 2-DE (two-dimensional electrophoresis) experiments.

Main Results:

Identified 2742 proteins in forager and nurse honeybee brains.
17% of identified proteins were differentially expressed between the two subcastes.
Nurse brains showed higher expression of proteins involved in translation, ribosomal structure, and biogenesis (14.5%) compared to foragers (1.8%).
Forager brains exhibited significantly higher expression of proteins related to energy production and conversion (17%) compared to nurses (0.6%).
Nine differentially expressed proteins were consistent between MudPIT and 2-DE experiments.

Conclusions:

Significant proteomic divergence exists between honeybee nurse and forager brains, reflecting their distinct physiological roles.
The findings highlight the specialized protein machinery supporting translation in nurses and energy metabolism in foragers.
MudPIT is a powerful technique for large-scale proteome mapping and identifying caste-specific protein expression in insects.