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Related Experiment Video

Updated: May 26, 2026

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
07:04

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Published on: November 9, 2014

Modified cytotoxicity evaluation of elastomeric impression materials while polymerizing with reduced exposure time.

Jae-Sung Kwon1, Sang-Bae Lee, Chong-Kwan Kim

  • 1Research Center for Orofacial Hard Tissue Regeneration, Yonsei University College of Dentistry, Seoul, Republic of Korea.

Acta Odontologica Scandinavica
|December 21, 2011
PubMed
Summary

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Cytotoxicity testing of dental impression materials should use the polymerizing (setting) form, not the set form. Shorter contact and extraction times (15-30 minutes) better reflect clinical use and yield significant differences in results.

Area of Science:

  • Biomaterials Science
  • Dental Materials Science
  • Toxicology

Background:

  • Cytotoxicity evaluation is crucial for assessing the biocompatibility of dental impression materials.
  • Traditional cytotoxicity tests use set impression materials with prolonged contact times (≥24 hours).
  • Clinical application involves contact with oral tissues during the polymerizing (setting) phase, typically under 10 minutes.

Purpose of the Study:

  • To investigate the differences in cytotoxicity results between polymerizing and set dental impression materials.
  • To compare cytotoxicity outcomes based on varying contact and extraction durations (15, 30 minutes vs. 12, 24 hours).

Main Methods:

  • Seven dental impression materials with diverse chemical compositions were evaluated.
  • In vitro cytotoxicity was assessed using extraction and direct contact tests.

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Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization
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Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization

Published on: July 3, 2018

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Last Updated: May 26, 2026

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
07:04

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Published on: November 9, 2014

Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization
08:02

Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization

Published on: July 3, 2018

  • Both polymerizing and set material forms were tested with contact/extraction times of 15, 30 minutes, 12, and 24 hours.

    • Main Results:

    • Significant differences (p < 0.05) in cell viability and proliferation were observed between polymerizing and set material forms.
    • Varying the duration of contact and extraction time also led to significant differences in results (p < 0.05).

    Conclusions:

    • Cytotoxicity evaluation of dental impression materials should utilize the polymerizing (setting) state.
    • Shorter contact and extraction times (15-30 minutes) are recommended as they more accurately reflect clinical scenarios.
    • These findings highlight the importance of testing conditions in determining the biocompatibility of dental materials.