Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Inflammatory Stress Response During Pregnancy as a Connecting Link in Intergenerational Risk Cascades.

Developmental psychobiology·2026

Same author

Detection of Posttraumatic Stress Disorder With Rest-Activity Data: Machine Learning Approach Using Wearable and Self-Report Data.

JMIR formative research·2026

Same author

Trauma, Stress, and Reproductive Health: A Bio-Psycho-Social Perspective on Violence Exposure in Immigrant Women.

Research in nursing & health·2026

Same author

Rural targeted messaging, multiplex salivary measurement, and rural equity in SARS-CoV-2 antibody testing: Protocol overview of a SeroNet investigation.

Contemporary clinical trials communications·2026

Same author

Daily discrimination and inflammation patterns among Black and Hispanic adolescents.

Psychoneuroendocrinology·2026

Same author

Timing of Prenatal Stress Exposure Predicts Infant Sympathetic Nervous System and Affective Responses.

Developmental psychobiology·2026

Search research articles

Related Experiment Video

Updated: May 26, 2026

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Published on: August 27, 2014

Assessing genetic polymorphisms using DNA extracted from cells present in saliva samples.

Zsofia Nemoda¹, Maria Horvat-Gordon, Christine K Fortunato

¹Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Tuzolto utca, Budapest, Hungary. zsofia.nemoda@eok.sote.hu

BMC Medical Research Methodology

|December 21, 2011

Summary

High-quality DNA for genetic analysis can be obtained from small whole saliva volumes. Collecting absorbent devices separately is recommended when using them for saliva DNA extraction.

More Related Videos

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Related Experiment Videos

Last Updated: May 26, 2026

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Published on: August 27, 2014

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Area of Science:

Genomics
Molecular Biology
Forensic Science

Background:

Advances post-Human Genome Project enable DNA extraction from whole saliva.
Previous studies lack detailed assessment of sample usability and environmental effects on saliva DNA quality.

Purpose of the Study:

Evaluate effects of sample volume, handling, storage, collection device, and sampling location on saliva DNA quantity, quality, and genetic assessment.

Main Methods:

Collected saliva from ten adults across five studies.
Tested varying saliva volumes (.10-1.0 ml), collection devices, oral locations, room temperature storage, and freeze-thaw cycles.
Performed genetic analyses on catechol-0-methyltransferase (COMT rs4680) and serotonin transporter (5-HTTLPR) genes.

Main Results:

Minimum .10 ml saliva yielded sufficient DNA (1.43 ± .77 μg) for accurate genotyping.
Collection devices retained 54-92% of DNA; 'adhered cell' extraction recovered this DNA with good quality.
Room temperature storage, freeze-thaw cycles, and sampling location had negligible effects on DNA quality and genetic analysis.

Conclusions:

At least .10 ml whole saliva is adequate for high-quality DNA extraction for genetic assays.
Recovered DNA supports over one hundred candidate gene assays per 10 ng.
Advise separate collection of absorbent devices to maximize nucleic acid recovery for genetic analysis.