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Related Experiment Video

Updated: May 26, 2026

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
12:31

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Published on: May 1, 2018

Rapid, multiplexed microfluidic phage display.

Kellye Cung1, Russell L Slater, Yue Cui

  • 1Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.

Lab on a Chip
|December 21, 2011
PubMed
Summary
This summary is machine-generated.

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A novel microfluidic chip enables rapid, high-throughput proteomic screening by identifying high-affinity phage-displayed peptides for multiple targets in a single round, accelerating discovery for disease markers and therapeutics.

Area of Science:

  • Proteomics and Molecular Biology
  • Biotechnology and Bioengineering

Background:

  • Phage display is a powerful technique for selecting peptides with specific binding affinities.
  • Current phage display methods are time-consuming and cumbersome, especially for multiple targets.
  • Automated, high-throughput screening methods are needed for advancing molecular interaction studies and disease marker discovery.

Purpose of the Study:

  • To develop and demonstrate a microfluidic chip for high-throughput, automated proteomic screening.
  • To enable the identification of high-affinity phage-displayed peptides for multiple targets in a single round.
  • To reduce the time and complexity associated with traditional phage display methods.

Main Methods:

  • Development of a novel microfluidic chip for phage display screening.

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Related Experiment Videos

Last Updated: May 26, 2026

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
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Published on: May 1, 2018

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06:30

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

  • Utilized the chip to screen for high-affinity phage-displayed peptides against multiple targets simultaneously.
  • Eliminated the need for bacterial infection in the screening process.

    • Main Results:

    • The microfluidic chip successfully identified high-affinity phage-displayed peptides for multiple targets in a single round.
    • The chip yielded known consensus sequences and identified novel sequences for clinically relevant targets.
    • Demonstrated significant time reduction and controlled assay conditions compared to traditional methods.

    Conclusions:

    • The developed microfluidic chip offers a rapid and efficient platform for proteomic screening.
    • This technology has the potential to accelerate the discovery of disease markers and therapeutic targets.
    • The easily-fabricated, disposable device can impact fundamental studies of molecular interactions and automated proteomic screening.