Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbe-Plant Interactions01:09

Microbe-Plant Interactions

Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flax rust infection transcriptomics reveals a transcriptional profile that may be indicative for rust Avr genes.

PloS one·2019
Same author

Characterisation of Stramenopile-specific mastigoneme proteins in Phytophthora parasitica.

Protoplasma·2018
Same author

Structural and functional insights into the modulation of the activity of a flax cytokinin oxidase by flax rust effector AvrL567-A.

Molecular plant pathology·2018
Same author

Phytophthora cinnamomi.

Molecular plant pathology·2017
Same author

Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini.

BMC genomics·2016
Same author

Quantifying the plant actin cytoskeleton response to applied pressure using nanoindentation.

Protoplasma·2016

Related Experiment Video

Updated: May 26, 2026

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
08:19

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

Confocal microscopy in plant-pathogen interactions.

Adrienne R Hardham1

  • 1Plant Science Division, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University, Canberra, ACT, Australia. Adrienne.Hardham@anu.edu.au

Methods in Molecular Biology (Clifton, N.J.)
|December 21, 2011
PubMed
Summary
This summary is machine-generated.

Confocal microscopy revolutionizes plant-pathogen studies by enabling high-resolution visualization of molecules and cellular components in intact tissues. This advanced imaging technique enhances understanding of plant defense mechanisms and pathogen infection strategies.

More Related Videos

Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens
06:12

Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens

Published on: September 15, 2023

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae
06:58

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

Published on: August 5, 2022

Related Experiment Videos

Last Updated: May 26, 2026

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
08:19

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens
06:12

Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens

Published on: September 15, 2023

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae
06:58

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

Published on: August 5, 2022

Area of Science:

  • Plant biology
  • Microscopy
  • Molecular biology

Background:

  • Plant-pathogen interactions are crucial for agriculture and ecosystem health.
  • Understanding molecular and cellular roles in these interactions is vital.
  • Previous imaging techniques had limitations in resolution and sample type.

Purpose of the Study:

  • To highlight the impact of confocal microscopy on plant-pathogen interaction research.
  • To explain the capabilities of confocal microscopy in visualizing cellular components.
  • To demonstrate its utility in studying plant defense mechanisms.

Main Methods:

  • Utilizing confocal microscopy for high-resolution imaging.
  • Visualizing fluorescent and fluorescently tagged molecules in fixed and living cells.
  • Capturing serial optical sections in the z-axis.
  • Applying computational image analysis techniques.

Main Results:

  • Confocal microscopy provides high-resolution visualization of molecules and cellular components.
  • It allows imaging in both single cells and intact plant tissues.
  • Image quality is improved by reducing out-of-focus light interference.
  • It enables detailed localization of molecules and interactions.

Conclusions:

  • Confocal microscopy has revolutionized plant-pathogen interaction studies.
  • It offers a powerful tool for localizing and studying molecular and cellular events.
  • This technique significantly advances the understanding of plant defense and pathogen strategies.