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Updated: May 26, 2026

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
Published on: June 19, 2013
Karyotyping methods for fungi
Rahim Mehrabi1, Masatoki Taga, Mostafa Aghaee
1Seed and Plant Improvement Institute, Karaj, Iran. Rahim.Mehrabi@spii.ir
Abstract:
Pulsed field gel electrophoresis enables separation of fungal chromosomes up to several megabases and is a worthwhile tool for fungal karyotyping. The germ tube burst method is a technique to separate fungal chromosomes of any size for chromosome number determination as well as in situ hybridization. Here we provide detailed protocols for both complementary methods that have many applications in fungal biology including chromosome size and chromosome number polymorphisms, and in situ localization of genes on chromosomes.
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