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[Evaluation of biofilm formation by Proteus mirabilis strains on the surface of different biomaterials by two
Joanna Kwiecińska-Piróg1, Tomasz Bogiel, Eugenia Gospodarek
1Katedra i Zakład Mikrobiologii, Collegium Medicum im. L. Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu.
Abstract:
Proteus sp. rods are opportunistic human pathogens. These microorganisms are mainly isolated from patients with urinary tract infections, particularly associated with using of biomaterials, on which surface they can form biofilm. The aim of our study was the estimation of Proteus mirabilis rods ability to form biofilm on the surface of 5 biomaterials (polychloride vinyl, silicone latex, polypropylene, polybutylen teraftalan and polyamide) using Richards' and quantitative method and comparison results of both methods. A total number of 84 P. mirabilis strains were included into the study. All of them were isolated in the Department of Clinical Microbiology University Hospital no. 1 of dr A. Jurasz Collegium Medicum of L. Rydygier in Bydgoszcz, Nicolaus Copernicus University in Toruń between 2005 and 2008. Examined P. mirabilis strains formed heavy biofilm with statistically significantly values on the surface of silicone latex than on polychloride vinyl and on polypropylene surface than polybutylen teraftalen or polyamide. High correlation of both methods was established. The Richards' method can be used to quick identification of P. mirabilis biofilm.
Insights
Proteus mirabilis bacteria form biofilms on biomaterials, especially silicone latex and polypropylene. Richards
Area of Science:
- Microbiology
- Biomaterials Science
Background:
- Proteus sp. are opportunistic human pathogens frequently linked to urinary tract infections.
- These bacteria can form biofilms on the surface of medical biomaterials, complicating infections.
- Understanding Proteus mirabilis biofilm formation on various biomaterials is crucial for infection control.
Purpose of the Study:
- To evaluate the biofilm-forming capacity of Proteus mirabilis on five different biomaterials.
- To compare the efficacy of Richards' method and a quantitative method for assessing biofilm formation.
- To determine which biomaterials are most susceptible to Proteus mirabilis biofilm colonization.
Main Methods:
- Tested 84 clinical isolates of Proteus mirabilis.
- Assessed biofilm formation on polychloride vinyl, silicone latex, polypropylene, polybutylene terephthalate, and polyamide.
- Employed both Richards' method and a quantitative method for biofilm assessment and compared results.
Main Results:
- Proteus mirabilis strains exhibited statistically significant heavy biofilm formation on silicone latex compared to polychloride vinyl.
- Significant heavy biofilm formation was observed on polypropylene compared to polybutylene terephthalate and polyamide.
- A high correlation was found between Richards' method and the quantitative method.
Conclusions:
- Silicone latex and polypropylene are highly susceptible to Proteus mirabilis biofilm formation.
- Richards' method is a reliable and quick technique for identifying Proteus mirabilis biofilm.
- Findings provide insights into biomaterial selection to mitigate Proteus-associated infections.
