[Genotyping methods of Listeria monocytogenes]

Listeriosis could occur as sporadic case as well as epidemic outbreak. For that reason it is important to subtype the Listeria monocytogenes strains using the fastest and most effective method. Genetic techniques are the most effective methods for differentiation of this species. Basing on latest available data eight selected subtyping genetic methods of Listeria monocytogenes strains were characterized: REA-PFGE, rybotyping, AFLP, PCR-RFLP, multiplex PCR, and methods from RAPD groups, such as AP-PCR, rep-PCR and ERIC -PCR. Analysis of available information allowed to assess that the most effective methods for L. monocytogenes typing are REA-PFGE and rybotyping. However, the rep-PCR and ERIC-PCR methods could be used in the routine Listeria monocytogenes diagnosis.