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Updated: May 26, 2026

Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models
Published on: April 26, 2024
Effect of extremely low frequency magnetic field on enamel microhardness in rats
1Department of Paediatric Dentistry, Marmara University, Istanbul, Turkey. bkargul@marmara.edu.tr
Aim:
The potential effects on human health of extremely low frequency magnetic field (ELF-MF) are of considerable concern. In the present study, it was investigated the effect of long term ELF-MF exposure on microhardness of rat teeth.
Materials And Methods:
Thirty male Sprague-Dawley rats were divided into three groups. The first and second experimental groups (n=10) were exposed to 100 μT and 500 μT ELF-MF during 10 months, 2 hours a day respectively. For the control group, nothing was applied to the rats and they completed their life cycle in the cage during the study period. After ELF-MF exposure, microhardness of enamel surface was determined for each group.
Results:
The decrease in microhardness in the second experimental group was found to be statistically significant compared to the control group (P <0.05). However, no statistical difference was found between the first and second experimental groups (P >0.05).
Conclusion:
From the results it can be concluded that 500 μT level of magnetic field strengths may have a certain negative effect on enamel mineralisation. Further investigations are necessary to analyse the effect of ELF-MF on teeth.

