Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Controlled-Potential Coulometry: Electrolytic Methods01:17

Controlled-Potential Coulometry: Electrolytic Methods

Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential ensures...
Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
Controlled-Current Coulometry: Overview01:27

Controlled-Current Coulometry: Overview

Controlled current coulometry, also known as amperostatic coulometry, is a technique used in electrochemical analysis to measure the quantity of a substance through the controlled passage of current. It involves the application of a constant current to an electrochemical cell containing the analyte of interest. As the current flows through the cell, the analyte undergoes a redox reaction at the electrode surface, resulting in a charge transfer. By monitoring the time required for a certain...
Voltammetric Techniques: Pulse Voltammetry01:17

Voltammetric Techniques: Pulse Voltammetry

Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...
Interfacial Electrochemical Methods: Overview01:06

Interfacial Electrochemical Methods: Overview

Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current passing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A disposable molecularly imprinted electrochemical sensing electrode for sub-picomolar histamine detection based on a Prussian blue-embedded imprinted polypyrrole coating on AuNPs@Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene nanocomposite.

Food chemistry·2026
Same author

Overcoming Limitations for Ultrasensitive <i>In Situ </i>pH Measurements in Marine Waters while Maintaining Traceability to Primary Standards.

Chimia·2026
Same author

Symmetrical pH Electrochemical Cell Coupled to Constant Potential Coulometry for Improved Sensitivity and Precision: Part 1. Fundamental Considerations.

ACS measurement science au·2026
Same author

Symmetrical pH Electrochemical Cell Coupled to Constant Potential Coulometry for Improved Sensitivity and Precision: Part 2. Submersible Probe for In Situ Measurements.

ACS measurement science au·2026
Same author

Flow injection amperometric sensor for sulfite detection in worldwide noodles using a novel redox composite of nickel hexacyanoferrate decorated on 3D mesoporous graphene aerogel.

Talanta·2025
Same author

Zero-current chronopotentiometry for wired biosensors.

Mikrochimica acta·2025

Related Experiment Video

Updated: May 26, 2026

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue
11:08

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue

Published on: September 5, 2015

Interference compensation for thin layer coulometric ion-selective membrane electrodes by the double pulse technique.

Ewa Grygolowicz-Pawlak1, Apon Numnuam, Panote Thavarungkul

  • 1Department of Inorganic, Analytical and Applied Chemistry, University of Geneva, Quai Ernest-Ansermet 30, CH-1211 Geneva, Switzerland.

Analytical Chemistry
|December 22, 2011
PubMed
Summary

A new double pulse method enhances ion-selective membrane sensors by reducing interference during coulometric detection. This technique improves operational selectivity, minimizing errors in real-world applications.

More Related Videos

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
09:18

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Published on: May 3, 2015

Related Experiment Videos

Last Updated: May 26, 2026

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue
11:08

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue

Published on: September 5, 2015

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
09:18

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Published on: May 3, 2015

Area of Science:

  • Analytical Chemistry
  • Electrochemistry
  • Membrane Science

Background:

  • Ion-selective membranes in coulometric detection offer recalibration-free sensing.
  • Analyte ions are removed via applied potential, with current integrated for quantification.
  • Exhaustive analyte removal demands high membrane selectivity to avoid interference.

Purpose of the Study:

  • To evaluate a double pulse protocol for reducing interference in coulometric detection.
  • To enhance the operational selectivity of ion-selective sensors.
  • To compensate for undesired interference in systems with limited membrane selectivity.

Main Methods:

  • Numerical simulation of a double pulse protocol for ions with the same charge.
  • Experimental validation using a calcium-selective membrane and tetraethylammonium as an interfering agent.
  • Comparison of results with uncompensated coulometry and direct potentiometry.

Main Results:

  • The double pulse protocol significantly reduces interference, improving operational selectivity by approximately 6-fold.
  • Numerical simulations confirmed the coulomb number reduction for systems with limited selectivity.
  • Experimental results showed favorable comparison to direct potentiometry after background compensation.

Conclusions:

  • The double pulse protocol effectively compensates for interference in coulometric detection.
  • This method enhances the practical applicability of ion-selective sensors in complex samples.
  • The technique offers a viable alternative for accurate ion quantification without frequent recalibration.