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Microarray analysis of differentially expressed microRNAs in allergic rhinitis
Yu Shaoqing1, Zhang Ruxin, Liu Guojun
1Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai, China.
Background:
Allergic rhinitis (AR) is a common disease characterized by chronic inflammation of the nasal mucosa, but we have not fully understood the mechanism responsible for the development of AR. MicroRNAs (miRNAs) are short endogenous noncoding RNAs regulating protein translation through a mechanism known as RNA interference. To understand the molecular mechanisms of miRNA involved in the pathogenesis of AR, expressed miRNAs in AR were investigated through genomewide microarray analysis.
Methods:
Mammalian miRNA microarrays containing whole human mature and precursor miRNA sequences were used for analyzing eight samples of nasal mucosa of AR and eight samples of nonallergic patients. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) of some different expressed miRNAs was used to confirm the array results.
Results:
The miRNA microarray chip analysis identified 421 miRNAs differentially expressed in the nasal mucosa of AR, and a total of 9 miRNAs were identified in the AR group with twofold change compared with control samples (p < 0.05). These included up-regulated miRNAs, hsa-hsa-miR-7, and hsa-miRPlus-E1194, and down-regulated miRNAs, hsa-miR-498, hsa-miR-187, hsa-miR-874, hsa-miR-143, hsa-miR-886-3p, hsa-miR-224, and hsa-miR-767-5p. RT-PCR results also confirmed that part of differentially expressed miRNAs as hsa-miR-224, hsa-miR-187, and hsa-miR-143 were down-regulated in AR.
Conclusion:
The report indicated that many miRNA expressions were altered in AR and differentially expressed miRNAs appear to be involved in the development of AR. The study of miRNAs may lead to a better understanding about the roles of identified miRNAs in the pathogenesis of AR; this would be considered in future therapeutic strategies.
Insights
This study identified altered microRNA (miRNA) expression in allergic rhinitis (AR). Specific miRNAs were found to be up-regulated or down-regulated, suggesting their involvement in AR development and potential therapeutic targets.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Allergic rhinitis (AR) involves chronic nasal inflammation with incompletely understood mechanisms.
- MicroRNAs (miRNAs) are key regulators of gene expression via RNA interference.
- Investigating miRNA expression in AR is crucial for understanding its pathogenesis.
Purpose of the Study:
- To identify differentially expressed miRNAs in the nasal mucosa of patients with allergic rhinitis.
- To explore the role of miRNAs in the molecular mechanisms underlying AR development.
Main Methods:
- Genome-wide microarray analysis of miRNA expression in nasal mucosa samples from AR patients and non-allergic controls.
- Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to validate array findings for specific miRNAs.
Main Results:
- Microarray analysis revealed 421 differentially expressed miRNAs in AR nasal mucosa.
- Nine miRNAs showed a twofold change (p < 0.05), including up-regulated hsa-miR-7 and hsa-miRPlus-E1194.
- Down-regulated miRNAs identified were hsa-miR-498, hsa-miR-187, hsa-miR-874, hsa-miR-143, hsa-miR-886-3p, hsa-miR-224, and hsa-miR-767-5p.
- RT-PCR confirmed down-regulation of hsa-miR-224, hsa-miR-187, and hsa-miR-143 in AR.
Conclusions:
- Altered miRNA expression is a significant feature of allergic rhinitis.
- Differentially expressed miRNAs are implicated in the pathogenesis of AR.
- Further study of these identified miRNAs could inform future therapeutic strategies for AR.
