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Published on: February 20, 2012
Development and characterization of polymorphic microsatellite markers for Conopholis americana (Orobanchaceae)
Anuar G Rodrigues1, Alison E L Colwell, Sasa Stefanovic
1Department of Biology, University of Toronto Mississauga, Ontario, Canada. anuar.rodrigues@utoronto.ca
Premise Of The Study:
Conopholis americana is an obligate root parasite with highly reduced morphology. To investigate the population structure, genetic diversity, and mating system of this predominantly eastern North American species, we developed polymorphic microsatellite markers for C. americana.
Methods And Results:
Using an enrichment cloning protocol, we report the isolation and characterization of 11 microsatellite markers. Product size varied from 198-370 bp. These loci show moderate levels of allelic variation (averaging 4.182 alleles per locus) and very low levels of heterozygosity (average observed heterozygosity = 0.054).
Conclusions:
These microsatellite markers will be useful in obtaining estimates of population-level genetic diversity and in phylogeographic studies of C. americana.
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