Related Experiment Video
Updated: May 26, 2026

Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality
Published on: April 7, 2023
Endospore production allows using spray-drying as a possible formulation system of the biocontrol agent Bacillus
V Yánez-Mendizabal1, I Viñas, J Usall
1IRTA, XaRTA-Post-harvest, 191 Rovira Roure, 25198, Lleida, Catalonia, Spain.
Abstract:
The role of endospore production by Bacillus subtilis CPA-8 on survival during spray-drying was investigated by comparison with a non-spore-forming biocontrol agent Pantoea agglomerans CPA-2. Endospore formation promoted heat resistance in CPA-8 depending on growth time (72 h cultures were more resistant than 24 h ones). The survival of CPA-8 and CPA-2 after spray-drying was determined after being grown in optimised media for 24 and 72 h. Spray-dried 72 h CPA-8 had the best survival (32%), while CPA-2 viability was less than 2%. CPA-8 survival directly related with its ability to produce endospores. Spray-dried CPA-8 reduced Monilinia fructicola conidia germination similarly to fresh cells, demonstrating that spray-drying did not adversely affect biocontrol efficacy. Endospore production thus improves CPA-8 resistance to spray-drying. These results can provide a reliable basis for optimising of the spray-drying formulation process for CPA-8 and other microorganisms.
More Related Videos
10:03Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy
Published on: November 30, 2017
09:38Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
Published on: January 21, 2016
Related Concept Videos
Production of Biopesticides
Endospores and Sporulation
Gene Regulation During Sporulation
Production of Antibiotics
Biological Methods for Microbial Control