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Microbial Biosensors01:17

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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
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Chemiluminescence-based biosensor for fumonisins quantitative detection in maize samples.

Mara Mirasoli1, Angela Buragina, Luisa Stella Dolci

  • 1Department of Pharmaceutical Sciences, Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.

Biosensors & Bioelectronics
|December 23, 2011
PubMed
Summary

A new portable biosensor enables rapid, ultrasensitive on-site detection of fumonisins in maize. This chemiluminescent immunoassay offers accurate quantification for improved food safety.

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Area of Science:

  • Analytical Chemistry
  • Biosensor Technology
  • Food Safety

Background:

  • Fumonisins (fumonisin B1+fumonisin B2) are mycotoxins commonly found in maize.
  • Accurate and rapid detection of fumonisins is crucial for ensuring food safety and preventing health risks.
  • Conventional methods for fumonisin detection can be time-consuming and require specialized laboratory equipment.

Purpose of the Study:

  • To develop a compact, portable, and ultrasensitive chemiluminescent biosensor for on-site quantification of fumonisins in maize.
  • To provide a rapid and accurate alternative to existing methods for fumonisin analysis.
  • To enable point-of-use applications for fumonisin detection in agricultural settings.

Main Methods:

  • Development of a competitive lateral flow immunoassay integrated with enzyme-catalyzed chemiluminescence detection.
  • Utilization of a portable charge-coupled device (CCD) camera in a contact imaging configuration for signal detection.
  • Implementation of a simple extraction procedure involving sample suspension in buffered solution and rapid heating to eliminate endogenous peroxidase activity.

Main Results:

  • Achieved a limit of detection of 2.5 μgL⁻¹ for fumonisins, with an analytical working range of 2.5-500 μgL⁻¹ (25-5000 μgkg⁻¹ in maize flour).
  • Demonstrated accurate and objective quantification using chemiluminescence detection, surpassing qualitative results from conventional colloidal gold-based assays.
  • Obtained high recovery rates (90-115%) in maize flour samples compared to LC-MS/MS analysis, with a total assay time of 25 minutes.

Conclusions:

  • The developed chemiluminescence immunochromatography-based biosensor is a rapid, low-cost, and portable testing solution.
  • This biosensor is suitable for point-of-use applications, enabling on-site fumonisin quantification in maize.
  • The technology offers a significant advancement for on-site food safety monitoring and risk assessment.