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Related Experiment Video

Updated: May 26, 2026

Vitrification of Ovarian Cortex Tissue to Achieve a Glassy State of Aggregation

Vitrification of Ovarian Cortex Tissue to Achieve a Glassy State of Aggregation

Published on: August 9, 2024

Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation.

Jacira Ribeiro Campos¹, Julio Cesar Rosa-e-Silva, Bruno Ramalho Carvalho

¹Universidade de São Paulo, Department of Obstetrics and Gynecology, Ribeirão Preto/SP, Brazil.

Clinics (Sao Paulo, Brazil)

|December 23, 2011

Summary

Cryopreservation of ovarian tissue preserves steroidogenic capacity but decreases follicular viability by approximately 30%. Short-term storage does not further compromise follicle integrity, indicating successful slow freezing protocols for ovarian tissue banking.

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Fertility Preservation in Patients with Severe Ovarian Dysfunction

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Published on: March 25, 2021

Area of Science:

Reproductive Biology
Cryobiology
Oncology

Background:

Ovarian tissue cryopreservation is crucial for fertility preservation in cancer patients.
Assessing the impact of storage duration on ovarian tissue quality is essential for optimizing fertility preservation techniques.

Purpose of the Study:

To evaluate the effect of storage duration (30 and 180 days) on cryopreserved ovarian tissue.
To compare follicular density, viability, and steroidogenic capacity between fresh and cryopreserved ovarian tissues.

Main Methods:

Ovarian biopsies were obtained from 17 fertile patients.
Tissue samples were divided into fresh (FG), cryopreserved for 30 days (G30), and cryopreserved for 180 days (G180).
Follicular density, viability, and steroidogenic capacity were assessed post-thaw.

Main Results:

Follicular density remained consistent across fresh and cryopreserved groups.
Follicular viability was significantly reduced in cryopreserved tissues (70.8%-78.4%) compared to fresh tissue (93.4%).
Steroidogenic capacity was maintained in cryopreserved ovarian tissue, with no significant reduction observed.

Conclusions:

Slow freezing protocols effectively preserve ovarian tissue's steroidogenic capacity.
While follicular viability decreases by ~30% post-cryopreservation, short-term storage (up to 180 days) does not further impair follicle integrity.
These findings support the efficacy of current ovarian cryopreservation methods for fertility preservation.