Related Experiment Video
Updated: May 26, 2026

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress
Published on: July 29, 2022
CGCDB: a web-based resource for the investigation of gene coexpression in CHO cell culture
Colin Clarke1, Padraig Doolan, Niall Barron
1National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland. colin.clarke@dcu.ie
Unlabelled:
Coexpression analysis is a powerful, widely used methodology for the investigation of underlying patterns in gene expression data. This "guilt-by-association" approach aims to find groups of genes with closely correlated expression profiles. Observation of consistent correlations across phenotypically diverse samples indicates that these genes have a shared function. We have recently described the application of weighted gene coexpression network analysis (WGCNA) to a 295 sample production CHO cell line microarray dataset and elucidated groups of genes related to growth rate and cell-specific productivity (Qp). In this study, we present the CHO gene coexpression database (CGCDB), a web-based system, designed specifically for researchers in the CHO community to provide user-friendly access to these gene-gene coexpression patterns. In addition to correlation between genes, the direct correlations between probesets and either growth rate or Qp are provided. Results are presented to the user via an interactive network diagram and in a downloadable tabular format. It is hoped that this resource will allow researchers to prioritize cell line engineering and/or biomarker candidates to enhance CHO-based cell culture for the production of biotherapeutics.
Availability:
www.cgcdb.org.
Related Concept Videos
Cell Specific Gene Expression
Cell Specific Gene Expression
Coordination of Gene Expression Processes in Bacteria
Combinatorial Gene Control
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Reporter Genes
Commonly used reporter...
Constitutive and Regulated Gene Expression

