Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...

G-Protein Gated Ion Channels

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision. The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory organs,...

DNA-only Transposons

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The role of microglial Tim-3 in neuroinflammation and functional recovery after spinal cord injury.

Frontiers in neurology·2026

Same author

Co-evolution and adaptive management of the water-sediment-ecology-socioeconomic nexus under climate-socioeconomic change.

Scientific reports·2026

Same author

Diagnostic test accuracy of the pattern electroretinogram for glaucoma: a systematic review and meta-analysis.

Canadian journal of ophthalmology. Journal canadien d'ophtalmologie·2026

Same author

Hippocampal REDD1 inhibition is critical for alleviating depressive-like behaviors.

Acta pharmacologica Sinica·2026

Same author

Perspectives from the 2024 International Consortium for Spinal Genetics, Development and Disease (ICSGDD).

Genetics in medicine open·2025

Same author

Bimodal Visualization of Mitochondrial Viscosity Remodeling, Morphological Dynamics and Interorganelle Networks in Acute Kidney Injury.

Analytical chemistry·2025

Search research articles

Related Experiment Video

Updated: May 26, 2026

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

Localized in vivo hyperpolarization transfer sequences.

Mor Mishkovsky¹, Tian Cheng, Arnaud Comment

¹Laboratory for Functional and Metabolic Imaging, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. mor.mishkovsky@epfl.ch

Magnetic Resonance in Medicine

|December 23, 2011

Summary

This study introduces a new method for enhanced in vivo detection of hyperpolarized carbon-13 (13C) and attached protons in the rat brain. This technique improves spectral resolution for studying 13C-acetate metabolism.

More Related Videos

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

Published on: October 19, 2009

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR

Published on: July 27, 2019

Related Experiment Videos

Last Updated: May 26, 2026

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

Published on: October 19, 2009

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR

Published on: July 27, 2019

Area of Science:

Magnetic Resonance Imaging
Metabolic Imaging
Biochemistry

Background:

Hyperpolarized agents offer enhanced sensitivity for in vivo metabolic studies.
Detecting short T1 (13)C signals and attached protons is challenging.
Improved spectral resolution is crucial for understanding metabolic pathways.

Purpose of the Study:

To develop and validate a novel in vivo method for enhanced detection of hyperpolarized substrates and metabolites.
To improve spectral resolution in (13)C-acetate metabolism studies.
To enable probing of additional molecular sites using indirect detection.

Main Methods:

Designed and performed in vivo localized and fully adiabatic homonuclear and heteronuclear polarization transfer experiments.
Utilized hyperpolarized sodium [1,2-(13)C(2)] and sodium [1-(13)C] acetate infusion in rat brains at 9.4 T.
Employed an indirect detection scheme to enhance signal acquisition.

Main Results:

Achieved highly enhanced in vivo detection of short-T1 (13)C signals and attached protons.
Demonstrated the capability to probe additional molecular sites in hyperpolarized substrates.
Observed improved spectral resolution in the context of (13)C-acetate metabolism.

Conclusions:

The presented method significantly enhances in vivo detection of hyperpolarized (13)C and protons.
This indirect detection scheme provides improved spectral resolution for metabolic studies.
The technique is valuable for investigating (13)C-acetate metabolism and related biochemical processes.