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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
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A new 'Linc' between noncoding RNAs and blood development.

Vikram R Paralkar1, Mitchell J Weiss

  • 1Division of Hematology/Oncology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.

Genes & Development
|December 23, 2011
PubMed
Summary
This summary is machine-generated.

Researchers discovered a novel long noncoding RNA (lncRNA), LincRNA-EPS, that promotes red blood cell formation by preventing cell death. This finding offers potential new treatments for anemia.

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RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
11:04

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Published on: May 19, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • Long noncoding RNAs (lncRNAs) are crucial regulators of diverse biological processes, including cell division, survival, and differentiation.
  • Understanding the specific roles of lncRNAs in cellular functions is essential for advancing biological knowledge.

Purpose of the Study:

  • To identify and characterize novel lncRNAs involved in erythropoiesis.
  • To elucidate the molecular mechanisms by which lncRNAs regulate red blood cell formation.

Main Methods:

  • Gene expression analysis
  • RNA sequencing
  • Apoptosis assays
  • In vivo studies in murine models

Main Results:

  • Discovery of LincRNA erythroid prosurvival (LincRNA-EPS), a murine lncRNA.
  • LincRNA-EPS was found to facilitate erythropoiesis by suppressing apoptosis.
  • Identification of a novel genetic pathway involving lncRNAs in red blood cell development.

Conclusions:

  • LincRNA-EPS plays a significant role in promoting red blood cell formation.
  • The discovery of LincRNA-EPS expands the known functions of lncRNAs.
  • This novel pathway presents a potential therapeutic target for treating anemias.