Related Experiment Video
Updated: May 26, 2026
07:46
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
Published on: April 3, 2017
Increased macrophage migration into adipose tissue in obese mice
Da Young Oh1, Hidetaka Morinaga, Saswata Talukdar
1Department of Medicine, University of California, San Diego, La Jolla, California, USA.
Diabetes
|December 23, 2011
Summary
This study introduces a novel method for tracking monocytes in vivo, revealing the CCR2/MCP-1 system
Area of Science:
- Immunology
- Metabolic Diseases
- Cell Biology
Background:
- Macrophage-mediated inflammation is central to insulin resistance.
- Initial monocyte migration to form tissue macrophages is not well understood.
Purpose of the Study:
- To develop and utilize a novel in vivo method for tracking monocyte migration.
- To elucidate the roles of the CCR2/MCP-1 system in macrophage recruitment and polarization.
Main Methods:
- Developed a method for in vivo tracking of fluorescently labeled (PKH26) blood monocytes.
- Utilized knockout mouse models (CCR2 KO, MCP-1 KO) to assess monocyte migration pathways.
- Quantified macrophage accumulation in adipose tissue, liver, and spleen.
Main Results:
- CCR2/MCP-1 signaling significantly impacts adipose tissue macrophage (ATM) and hepatic macrophage accumulation.
- Obesity dramatically increases ATM accumulation, independent of monocyte origin.
- Recruited ATMs in obese adipose tissue polarize from M2-like to M1-like states.
Conclusions:
- The CCR2/MCP-1 system is crucial for monocyte recruitment to adipose tissue and dominant for liver macrophage appearance.
- Increased pro-inflammatory ATMs in obesity result from tissue-derived signals, not pre-programmed monocytes.
- Understanding these mechanisms is key for targeting insulin resistance.
