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Related Experiment Video

Updated: May 26, 2026

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

Brucellosis and type IV secretion.

Maarten F de Jong¹, Renee M Tsolis

¹Department of Medical Microbiology & Immunology, University of California, Davis, CA, USA.

Future Microbiology

|December 24, 2011

Summary

This summary is machine-generated.

Brucellosis, a zoonotic infection caused by Brucella bacteria, often persists long-term in hosts. This review details the Brucella type IV secretion system, a key factor in bacterial survival and virulence.

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Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

Published on: October 13, 2015

Area of Science:

Microbiology
Immunology
Infectious Diseases

Background:

Brucellosis is a significant global zoonotic disease caused by Brucella bacteria, affecting both animals and humans.
Brucella species are intracellular pathogens known for their ability to evade host immune responses and establish persistent infections.
The type IV secretion system (T4SS) is a crucial virulence factor enabling Brucella survival within host cells.

Purpose of the Study:

To provide a comprehensive review of the Brucella type IV secretion system.
To detail the architecture and regulation of the Brucella T4SS.
To highlight newly identified effector substrates translocated by the T4SS into host cells.

Main Methods:

Literature review of existing research on Brucella T4SS.
Analysis of studies detailing T4SS structure and genetic regulation.
Examination of proteomic and genetic data identifying T4SS effector proteins.

Main Results:

The Brucella T4SS is essential for intracellular bacterial survival and pathogenesis.
Detailed understanding of the T4SS components, assembly, and regulatory mechanisms is emerging.
Several novel effector proteins translocated by the T4SS have been identified, contributing to host cell manipulation.

Conclusions:

The Brucella T4SS is a complex and vital system for establishing and maintaining infection.
Further research into T4SS effectors may reveal new therapeutic targets for brucellosis.
Understanding T4SS function is critical for developing strategies to combat this widespread zoonotic disease.