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Depth profiling of photothermal compound concentrations using phase sensitive optical coherence tomography.

Guangying Guan¹, Roberto Reif, Zhihong Huang

¹University of Washington, Department of Bioengineering, 3720 15th Avenue N. E., Seattle, Washington 98195, USA.

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|December 24, 2011

Summary

A new model quantifies photothermal compound concentration in turbid media using optical coherence tomography. This method accurately maps light-to-heat converters in various phantom models.

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Area of Science:

Biomedical Optics
Photothermal Imaging
Optical Coherence Tomography

Background:

Accurate quantification of photothermal agents is crucial for applications like photothermal therapy.
Turbid media present challenges for optical imaging due to light scattering.
Developing models to predict agent concentration in complex environments is essential.

Purpose of the Study:

To develop and validate a model for determining photothermal compound concentration as a function of depth in turbid media.
To integrate a pump-laser heating system with a phase-sensitive spectral domain optical coherence tomography (SD-OCT) system.
To empirically determine model coefficients and validate the model's predictive capability.

Main Methods:

A theoretical model was derived to describe photothermal compound concentration.
An 808 nm pump laser modulated at 400 Hz was used for heating.
Phase-sensitive spectral domain optical coherence tomography (SD-OCT) detected temperature-induced optical path length changes.
Model coefficients were determined using solid homogeneous gel phantoms.
Model validation was performed using thick single and double-layer solid phantoms.

Main Results:

A validated model was established to quantify photothermal compound concentration versus depth.
The system successfully detected temperature changes induced by photothermal compounds.
Reconstruction of photothermal compound concentration was achieved in complex phantom models.

Conclusions:

The developed model provides a reliable method for quantifying photothermal compound concentration in turbid media.
The combination of photothermal excitation and SD-OCT is effective for this application.
This approach has potential for in vivo applications requiring precise localization of photothermal agents.