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Updated: May 26, 2026
Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
Published on: July 12, 2022
Kinetic tracking of therapy-induced senescence using the real-time cell analyzer single plate system
Timo Weiland1, Alexander Berger, Frank Essmann
1Department of Internal Medicine I, Medical University Clinic, Tuebingen, Germany.
Abstract:
In recent years, terminal growth arrest, that is, senescence, especially therapy-induced senescence (TIS), has become a major subject in cancer research and several fields of life sciences. Senescence is characterized by a specific set of morphological and biochemical changes. However, methods that evidence senescence induction are still very limited and show large variation between individual examiners. Most notably, these assays are classical endpoint assays, and, therefore, screening for senescence is time consuming and expensive. Here, we describe an efficient, simple, and objective method to screen for TIS over time by modifying the Real-Time Cell Analyzer SP system, thus enabling to pin point the induction of senescence. This method continuously detects the cell's impedance in each well of a 96-microwell plate that allows to observe increment of cell size, a hallmark feature of cellular senescence. This technique is suitable for high-throughput TIS screening by measuring several compounds, small molecules, and/or cell lines simultaneously.
Insights
This study introduces a new, efficient method to screen for therapy-induced senescence (TIS) by continuously monitoring cell impedance. This technique allows for real-time observation of cell size increases, a key marker of senescence.
Area of Science:
- Oncology
- Cell Biology
- Biotechnology
Background:
- Cellular senescence, particularly therapy-induced senescence (TIS), is a critical area in cancer research.
- Current methods for detecting senescence are limited, often endpoint-based, time-consuming, and expensive.
- There is a need for efficient and objective assays to screen for TIS.
Purpose of the Study:
- To develop an efficient, simple, and objective method for screening therapy-induced senescence (TIS) over time.
- To enable real-time pinpointing of senescence induction.
- To facilitate high-throughput screening of compounds and cell lines for TIS.
Main Methods:
- Modification of the Real-Time Cell Analyzer SP system.
- Continuous monitoring of cell impedance in 96-microwell plates.
- Utilizing cell size increment, a hallmark of senescence, as a detection parameter.
Main Results:
- The developed method allows for continuous, real-time detection of senescence induction.
- The technique objectively measures cell size increase, a key feature of senescence.
- The method is suitable for high-throughput screening of multiple compounds and cell lines simultaneously.
Conclusions:
- This novel impedance-based method provides an efficient, simple, and objective approach to screen for TIS.
- The real-time monitoring capability facilitates precise determination of senescence induction.
- The high-throughput compatibility makes this technique valuable for drug discovery and cancer research.
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