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Related Concept Videos

Measurement of Bioavailability: Pharmacodynamic Methods01:20

Measurement of Bioavailability: Pharmacodynamic Methods

Pharmacodynamic methods provide insights into a drug's effects on physiological processes over time and play a crucial role in understanding bioavailability and therapeutic efficacy. These methods can be broadly classified into acute pharmacological and therapeutic response approaches, each with distinct mechanisms and applications.The acute pharmacological response method directly correlates a drug's physiological effects, such as ECG or pupil diameter changes, to its time course in the body.
Measurement of Bioavailability: Pharmacokinetic Methods01:30

Measurement of Bioavailability: Pharmacokinetic Methods

Pharmacokinetics is a vital branch of pharmacology that examines how drugs are absorbed, distributed, metabolized, and excreted by the body. Two key methodologies in pharmacokinetics are plasma drug concentration studies and urinary drug excretion analyses, both of which provide critical insights into a drug's therapeutic efficacy and bioavailability.Plasma Drug Concentration-Time StudiesPlasma drug concentration-time studies involve analyzing blood samples at specific intervals to quantify...

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HPLC/UV or bioassay: two valid methods for posaconazole quantification in human serum samples.

E Cendejas-Bueno1, A Forastiero, J L Rodriguez-Tudela

  • 1Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
|December 24, 2011
PubMed
Summary

Two methods for quantifying posaconazole in serum were developed and validated. High-performance liquid chromatography (HPLC)/UV is accurate and fast for reference labs, while bioassay is a simpler alternative for clinical monitoring.

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Area of Science:

  • Analytical Chemistry
  • Clinical Pharmacology

Background:

  • Posaconazole is a vital antifungal agent requiring therapeutic drug monitoring.
  • Accurate quantification of posaconazole in serum is crucial for optimizing patient outcomes.
  • Existing quantification methods may have limitations in terms of accessibility or throughput.

Purpose of the Study:

  • To develop and validate two distinct methods for posaconazole serum quantification: HPLC/UV and bioassay.
  • To compare the performance characteristics of both methods.
  • To assess their applicability in analyzing patient samples.

Main Methods:

  • Development and validation of a High-Performance Liquid Chromatography with Ultraviolet detection (HPLC/UV) method.
  • Development and validation of a microbiological bioassay method.
  • Application of both validated methods to analyze 61 trough serum samples from posaconazole-treated patients.

Main Results:

  • Both HPLC/UV and bioassay methods were validated according to international guidelines.
  • A strong correlation was observed between the results obtained from both methods.
  • The HPLC/UV method demonstrated superior accuracy, precision, and speed (11 min run time) within an analytical range of 0.125-16 μg/mL.
  • The bioassay method provided sufficient accuracy and precision for therapeutic drug monitoring, with a similar analytical range.

Conclusions:

  • The HPLC/UV method is a valuable tool for reference laboratories handling large sample volumes due to its accuracy, precision, and efficiency.
  • The bioassay method offers a simpler, viable alternative for posaconazole monitoring in clinical laboratories lacking specialized chromatographic facilities.
  • Both methods are reliable for posaconazole quantification in patient serum samples.