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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...
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Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...

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Related Experiment Video

Updated: May 26, 2026

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
09:57

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Published on: July 12, 2018

Flow cytometry for fast microbial community fingerprinting.

Karen De Roy1, Lieven Clement, Olivier Thas

  • 1Laboratory of Microbial Ecology and Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium.

Water Research
|December 24, 2011
PubMed
Summary
This summary is machine-generated.

A new flow cytometry method rapidly fingerprints microbial communities in water. This approach offers a faster, objective alternative to traditional techniques for water quality assessment.

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Last Updated: May 26, 2026

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Area of Science:

  • Microbiology
  • Analytical Chemistry
  • Environmental Science

Background:

  • Microbial characterization of water is crucial for food safety, beverage production, and wastewater management.
  • Traditional methods for microbial analysis are often slow and require extensive labor.
  • Objective and rapid techniques are needed for efficient water microbial community assessment.

Purpose of the Study:

  • To develop a fast and objective flow cytometry-based method for comparing microbial communities in water.
  • To establish a novel statistical pipeline for analyzing flow cytometric data.
  • To demonstrate the utility of this method for discriminating and monitoring aquatic microbial communities.

Main Methods:

  • Generation of microbial community fingerprint data using flow cytometry.
  • Application of a novel statistical pipeline for analyzing flow cytometric data.
  • Validation of the method on drinking water samples and under varying environmental conditions.

Main Results:

  • The flow cytometry approach successfully discriminated between different brands of drinking water.
  • The method effectively detected changes in microbial community composition due to environmental factors.
  • The developed method provides rapid and objective microbial fingerprinting capabilities.

Conclusions:

  • Flow cytometry offers a rapid and objective method for microbial community fingerprinting in aquatic samples.
  • This technique can serve as a valuable tool for detecting shifts in microbial communities.
  • The approach has broad applicability in water quality monitoring and management.