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Updated: May 26, 2026
10:02
Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
Published on: October 23, 2016
Lessons from yeast for clathrin-mediated endocytosis
Douglas R Boettner1, Richard J Chi, Sandra K Lemmon
1Department of Molecular and Cellular Pharmacology (R-189), Miller School of Medicine, University of Miami, Miami, Florida 33101, USA.
Nature Cell Biology
|December 24, 2011
Summary
Clathrin-mediated endocytosis (CME) is crucial for cell surface protein uptake. This review details vesicle formation, from initiation to uncoating, using budding yeast as a model system.
Area of Science:
- Cell Biology
- Molecular Biology
Background:
- Clathrin-mediated endocytosis (CME) is the primary route for internalizing cell surface membrane proteins.
- Extensive research has elucidated the intricate process of clathrin-coated vesicle formation.
- Live-cell imaging offers dynamic insights into CME dynamics.
Purpose of the Study:
- To review the molecular mechanisms of clathrin-coated vesicle formation.
- To highlight key findings from studies utilizing budding yeast as a model system.
- To trace the CME process from vesicle initiation to uncoating.
Main Methods:
- Literature review focusing on budding yeast as a model organism.
- Analysis of studies employing live-cell imaging techniques.
- Synthesis of research on the molecular machinery of CME.
Main Results:
- Budding yeast serves as an evolutionary conserved model for studying CME.
- Key molecular players and sequential steps in vesicle formation have been identified.
- The process involves coordinated actions from initiation to the final uncoating stage.
Conclusions:
- Clathrin-mediated endocytosis is a fundamental cellular process.
- Budding yeast provides valuable insights into the conserved mechanisms of CME.
- Understanding CME dynamics is essential for cell surface regulation.
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