Related Experiment Video
Updated: May 26, 2026

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
Published on: October 23, 2016
Bridging membrane and cytoskeleton dynamics in the secretory and endocytic pathways
Mihaela Anitei1, Bernard Hoflack
1Biotechnology Centre, Technische Universität Dresden, Tatzberg 47/49, 01307 Dresden, Germany.
Abstract:
Transport carriers regulate membrane flow between compartments of the secretory and endocytic pathways in eukaryotic cells. Carrier biogenesis is assisted by microtubules, actin filaments and their associated motors that link to membrane-associated coats, adaptors and accessory proteins. We summarize here how the biochemical properties of membranes inform their interactions with cytoskeletal regulators. We also discuss how the forces generated by the cytoskeleton and motor proteins alter the biophysical properties and the shape of membranes. The interplay between the cytoskeleton and membrane proteins ensures tight spatial and temporal control of carrier biogenesis, which is essential for cellular homeostasis.
Related Concept Videos
Cytoskeletal Coordination in Cell Migration
Assembly of the Lipid Bilayer in the ER
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
Mechanisms of Membrane-bending
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
ER Retrieval Pathway
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
Role of Septins
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
Assembly of Complex Microtubule Structures

