Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparing ultrasound-derived fat fraction and CT for diagnosing hepatic steatosis: an MRI-PDFF reference study.

Frontiers in medicine·2026
Same author

KIT and FLT3-ITD mutations do not predict outcomes in pediatric core-binding factor acute myeloid leukemia: findings from the C-HUANAN-AML-15 multicenter cohort study.

Annals of hematology·2026
Same author

Effect of delayed cord clamping on maternal and neonatal outcomes during cesarean delivery in twin pregnancies.

BMC pregnancy and childbirth·2026
Same author

Integrated Molecular Risk Stratification and Measurable Residual Disease-Guided Consolidation Improve Outcomes in Pediatric Non-Down Syndrome Acute Megakaryoblastic Leukemia.

Cancer medicine·2026
Same author

Alkali metal N-heteroarenes as a new family of luminescent materials.

Nature communications·2026
Same author

Driving Efficient Tandem Catalysis by Coupling Distinct Active Sites within a Ternary Hydride Lattice.

Journal of the American Chemical Society·2026

Related Experiment Video

Updated: May 26, 2026

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

Lysine methylation: beyond histones.

Xi Zhang1, Hong Wen, Xiaobing Shi

  • 1Department of Biochemistry & Molecular Biology, University of Texas MD Anderson Cancer Center, Houston, 77030, USA.

Acta Biochimica Et Biophysica Sinica
|December 24, 2011
PubMed
Summary
This summary is machine-generated.

Lysine methylation is a common posttranslational modification (PTM) in non-histone proteins, regulating diverse cellular processes. This PTM, similar to acetylation and phosphorylation, offers new insights into epigenetic regulation.

More Related Videos

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
11:06

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

Published on: December 29, 2017

Analysis of Histone Antibody Specificity with Peptide Microarrays
09:47

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

Related Experiment Videos

Last Updated: May 26, 2026

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
11:06

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

Published on: December 29, 2017

Analysis of Histone Antibody Specificity with Peptide Microarrays
09:47

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

Area of Science:

  • Epigenetics and Molecular Biology
  • Posttranslational Modifications
  • Chromatin Regulation

Background:

  • Posttranslational modifications (PTMs) like acetylation, methylation, phosphorylation, and ubiquitylation are crucial for chromatin dynamics.
  • The 'histone code' describes how combinations of PTMs on histones regulate DNA at the epigenetic level.
  • While phosphorylation, ubiquitylation, and acetylation are well-studied, lysine methylation on non-histone proteins remains less explored.

Purpose of the Study:

  • To review the current understanding of lysine methylation on non-histone proteins.
  • To highlight the regulatory roles of lysine methylation in various cellular functions.
  • To propose lysine methylation as a common PTM.

Main Methods:

  • Literature review of studies on lysine methylation of non-histone proteins.
  • Analysis of reported functions and regulatory mechanisms.
  • Comparison with other well-established PTMs.

Main Results:

  • Lysine methylation occurs on numerous non-histone proteins, impacting protein interactions, stability, and localization.
  • Evidence suggests lysine methylation regulates diverse cellular processes beyond chromatin.
  • The number of identified non-histone proteins undergoing lysine methylation is growing.

Conclusions:

  • Lysine methylation is a prevalent and significant posttranslational modification on non-histone proteins.
  • This PTM plays a critical role in regulating protein function and cellular processes.
  • Lysine methylation is proposed to be as common and functionally important as phosphorylation and acetylation.