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Related Concept Videos

Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Gene Regulation in Microbial Communities: Quorum Sensing

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Related Experiment Video

Updated: May 26, 2026

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes
08:34

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes

Published on: September 27, 2018

Conditional gene expression in Mycobacterium abscessus.

Mélanie Cortes1, Anil Kumar Singh, Jean-Marc Reyrat

  • 1Université Paris Descartes, Faculté de médecine, Paris, France.

Plos One
|December 24, 2011
PubMed
Summary
This summary is machine-generated.

Developing new genetic tools for Mycobacterium abscessus is crucial for understanding its virulence. This study demonstrates an effective conditional gene expression system, TetR/PipOFF, for both smooth and rough morphotypes of this emerging pathogen.

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Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos

Published on: September 9, 2015

Area of Science:

  • Microbiology
  • Molecular Biology
  • Pathogen Genetics

Background:

  • Mycobacterium abscessus is an emerging pathogen causing various infections.
  • Distinct smooth (S) and rough (R) morphotypes exist, with R associated with higher pathogenicity.
  • Limited genetic tools, particularly for homologous recombination and conditional gene expression, hinder M. abscessus virulence studies.

Purpose of the Study:

  • To evaluate the potential of a conditional gene expression system (TetR/PipOFF) for genetic studies in M. abscessus.
  • To adapt and validate the TetR/PipOFF system for both S and R morphotypes of M. abscessus.
  • To demonstrate the system's efficacy in controlling the expression of an essential gene.

Main Methods:

  • Adaptation of the TetR/PipOFF system for M. abscessus.
  • Application of the system for conditional gene expression of the essential gene fadD32.
  • Testing the system's efficiency in both S and R morphotypes.

Main Results:

  • The adapted TetR/PipOFF system proved effective for conditional gene expression in M. abscessus.
  • Inhibition of fadD32 expression was achieved in both S and R morphotypes.
  • The system showed marginally better efficiency in the R morphotype.

Conclusions:

  • Conditional gene expression using the developed TetR/PipOFF system is effective in both S and R M. abscessus.
  • This system represents a valuable new approach for future genetic research on M. abscessus virulence.
  • The findings facilitate deeper investigation into the mechanisms underlying M. abscessus pathogenesis.