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Isolation of a Friend recombinant polytropic virus with a T-cell-restricted leukemogenicity
V Della-Valle1, F Wendling, M Sitbon
1U-301 INSERM, Institute de Génétique Moléculaire, Paris, France.
Abstract:
Hematopoietic malignant cells of various types were isolated from ICFW mice inoculated as newborn or adult with F-MuLV and grafted into recipient mice. After repeated in vivo cell transplants, several recombinant polytropic viruses (also termed MCF or dualtropic viruses) were isolated from tumors by limiting dilution. Two virus isolates designated RA1-17 and EA1-17 were recovered from the spleen and the omentum, respectively, of the same grafted animal. When inoculated into newborn mice, RA1-17 induced erythroblastosis similar to that induced by other Friend recombinant polytropic viruses. Remarkably, EA1-17 induced T-cell leukemia after a short latency. To our knowledge, this is the first description of a polytropic recombinant virus with a T-cell tropism isolated after inoculation of F-MuLV.
Insights
Researchers isolated novel polytropic viruses from F-MuLV infected mice. One isolate, EA1-17, uniquely caused T-cell leukemia in recipient mice, offering new insights into viral oncogenesis.
Area of Science:
- Virology
- Oncology
- Immunology
Background:
- Friend Murine Leukemia Virus (F-MuLV) is known to cause hematopoietic malignancies.
- Recombinant polytropic viruses (MCF or dualtropic viruses) can arise during F-MuLV infection.
- Understanding viral tropism is crucial for identifying oncogenic mechanisms.
Purpose of the Study:
- To isolate and characterize novel recombinant polytropic viruses from F-MuLV infected mice.
- To investigate the oncogenic potential and tropism of these isolated viruses.
Main Methods:
- Isolation of hematopoietic malignant cells from F-MuLV inoculated mice.
- In vivo cell transplantation and serial passaging in recipient mice.
- Isolation of recombinant polytropic viruses using limiting dilution.
- Inoculation of isolated viruses into newborn mice to assess pathogenicity and tropism.
Main Results:
- Several recombinant polytropic viruses were successfully isolated from tumors.
- Two isolates, RA1-17 and EA1-17, were obtained from the same animal.
- RA1-17 induced erythroblastosis, consistent with known Friend recombinant polytropic viruses.
- EA1-17 demonstrated a novel T-cell leukemia tropism with a short latency period.
Conclusions:
- The isolation of EA1-17 represents the first polytropic recombinant virus with T-cell tropism linked to F-MuLV infection.
- This finding expands the known spectrum of F-MuLV-induced leukemias.
- These novel viral isolates provide valuable tools for studying viral oncogenesis and host-pathogen interactions.